Application

Research CategorySignaling

Anti-MCRS1, clone DON193B/C9, Cat. No. MABS1268, is a rat monoclonal antibody that detects Microspherule protein 1 and has been tested for use in Western Blotting.

Western Blotting Analysis: A representative lot detected MCRS1 in human tonsil, human spleen, HeLa cell, and Ramos cell lysates (Courtesy of Giovanna Roncador, Ph.D., CNIO, Madrid, Spain).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Microspherule protein 1 (UniProt: Q96EZ8; also known as 58 kDa microspherule protein, Cell cycle-regulated factor p78, INO80 complex subunit J, MCRS1) is encoded by the MCRS1 (also known as INO80Q, MSP58) gene (Gene ID: 10445) in human. MCRS1 is a nucleolar protein that is detected in testis, and at lower levels in spleen, thymus, prostate, uterus, small intestine, colon and leukocytes. Its highest levels are reported in the early S phase and it becomes undetectable in the G2 phase. Four isoforms of MCRS1 have been reported that are produced by alternative splicing. MCRS1 contains a nucleolar localization motif (aa 113-123), a coiled-coil domain (aa 301-335), and a forkhead associated (FHA) domain (aa 363-419). MCRS1 exhibits transforming activity, which is suppressed by phosphatase and tensin homologue (PTEN). It can modulate the transcription repressor activity of DAXX by recruiting it to the nucleolus. MCRS1 forms a complex with Mi-2 beta, and RET finger protein (RFP) and co-localize in the Nucleolus. This complex activates rRNA promoter activity. (Ref.: Shimono, K., et al. (2005). J. Biol. Chem. 280(47); 39436-39447).

Immunogen

A mixture of GST-tagged full length recombinant human Microspherule protein 1 and GST-tagged C- and N-terminal recombinant fragments of human Microspherule protein 1.

Epitope: unknown

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G purified

Format: Purified

Quality

Evaluated by Western Blotting in HCT116 cell lysate.

Western Blotting Analysis: 4 µg/mL of this antibody detected MCRS1 in 10 µg of HCT116 cell lysate.

Specificity

Clone DON193B/C9 detects Microspherule protein 1 in human cells.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~52 kDa observed; 51.80 kDa calculated. Uncharacterized bands may be observed in some lysate(s).